KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit anti-HA | Abcam | Cat#: ab9110, RRID:AB_307019 |
| Rat anti-HA | Roche | Cat#: 11867423001, RRID:AB_390918 |
| Chicken anti-keratin 5/14 | Generated in the Lechler Lab | N/A |
| Rabbit anti-keratin 10 | BioLegend | Cat#: 905401, RRID:AB_2565049 |
| Rat anti-BrdU | Abcam | Cat#: ab6326, RRID:AB_305426 |
| Rat anti-β4 integrin | BD Biosciences | Cat#: 553745, RRID:AB_395027 |
| Rabbit anti Myosin IIA | Biolegend | Cat#: 909801, RRID:AB_2565100 |
| Rabbit anti Myosin IIB | Biolegend | Cat#: 909901, RRID:AB_2565101 |
| Rabbit anti Myosin IIC | Biolegend | Cat#: 919201, RRID:AB_2565209 |
| Rabbit anti α-catenin | Sigma-Aldrich | Cat#: C2081, RRID:AB_476830 |
| Rat anti-α−18 | gift from Akira Nagafuchi | (Yonemura et al., 2010) |
| Mouse anti Vinculin | Sigma-Aldrich | Cat#: V9131, RRID:AB_477629 |
| Rabbit anti-YAP/TAZ | Cell Signaling Technology | Cat#: 8418, RRID:AB_10950494 |
| Goat anti-LHX2 | Santa Cruz Biotechnology | Cat#: sc-19344, RRID:AB_2135660 |
| Rabbit anti-SOX9 | Millipore | Cat#: AB5535, RRID:AB_2239761 |
| Goat anti-EDAR | EDAR | Cat#: AF745, RRID:AB_355565 |
| Rabbit anti-LEF1 | Cell Signaling Technology | Cat#: 2230, RRID:AB_823558 |
| Mouse anti-E-cadherin | BD Biosciences | Cat#: 610182, RRID:AB_397581 |
| Rabbit anti-c-Kit | Cell Signaling Technology | Cat#: 3074, RRID:AB_1147633 |
| Mouse anti-Desmoglein 1 | BD Biosciences | Cat#: 610273, RRID:AB_2093422 |
| Mouse anti-Desmoplakin | Millipore | Cat# CBL173, RRID:AB_93346 |
| Phalloidin-TRITC | Sigma-Aldrich | Cat#: P1951, RRID:AB_2315148 |
| Alexa Fluor 488 Phalloidin | Invitrogen | A12379 |
| Rabbit anti-GAPDH | Abcam | Cat#: ab9485, RRID:AB_307275 |
| IRDye 680RD Donkey anti-Rabbit IgG | LI-COR Biosciences | Cat#: 926–68073, RRID:AB_10954442 |
| IRDye 800CW Goat anti-Rabbit IgG | LI-COR Biosciences | Cat#: 925–32211, RRID:AB_2651127 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| BrdU | Sigma-Aldrich | Cat#:B5002-100MG |
| Y-27632 2HCl | Selleckchem | Cat#: S1049 |
| Blebbistatin | Sigma-Aldrich | Cat#: B0560 |
| Verteporfin | VWR/US pharm conv | Cat#: 1711461 |
| Doxycycline | Sigma-Aldrich | Cat#:D9891–1G |
| Fetal Bovine Serum | Mediatech | Cat#: 35–010-CV |
| Penicillin/Streptomycin | Gibco | Cat#:10378016 |
| DMEM | Gibco | Cat#: No. 11965 |
| Dispase II | Roche | Cat#: 4942078001 |
| Trypsin-EDTA (0.25%) | Gibco | Cat#: 25200056 |
| Versene Solution | Gibco | Cat#: 15040066 |
| Deoxyribonuclease I | Sigma-Aldrich | Cat#:D5025–15KU |
| Propidium iodide solution | Sigma-Aldrich | Cat#: P4864 |
| Albumin bovine serum | Sigma-Aldrich | Cat#:A7906–100g |
| Donkey serum | Sigma-Aldrich | Cat#: D9663 |
| Goat Serum | Gibco | Cat#: 16210–064 |
| Critical Commercial Assays | ||
| RNAeasy Mini kit | Qiagen | Cat#: 74104 |
| RNase-Free DNase Set | Qiagen | Cat#: 79254 |
| TransIT-LT1 Transfection Reagent | Mirus | Cat#: MIR 2300 |
| Deposited Data | ||
| Raw and analyzed data | This paper | GEO:GSE158786 |
| Experimental Models: Cell Lines | ||
| keratinocytes | This paper | N/A |
| Skin explants | This paper | N/A |
| Experimental Models: Organisms/Strains | ||
| Mouse: K10-rtTA | (Muroyama and Lechler, 2017) | N/A |
| Mouse: TRE-Spastin | (Muroyama and Lechler, 2017) | N/A |
| Mouse: Involucrin-tTA | (Jaubert et al., 2004) | N/A |
| Mouse: TRE-Arhgef11CA | This paper | N/A |
| Mouse: TRE-H2BGFP | Jackson Laboratories | Stock#: 005104 |
| Mouse: Rosa-mT/mG | Jackson Laboratories | Stock#: 007576 |
| Mouse: CMV-Cre | Jackson Laboratories | Stock#: 006054 |
| Mouse: mGFP | This paper | N/A |
| Mouse: Tyr-Cre | Jackson Laboratories | Stock#: 029788 |
| Mouse: Krt14Cre | Jackson Laboratories | Stock#: 018964 |
| Mouse: Rosa26-LSL-H2B mCherry | Jackson Laboratories | Stock#: 023139 |
| Mouse: E-cadherin-mCFP | Jackson Laboratories | Stock#: 016933 |
| Mouse: CD1 IGS | Charles River | Strain code: 022 |
| Oligonucleotides - See Table 1 | ||
| Recombinant DNA | ||
| Plasmid: pTRE2-HA-Arhgef11CA | This paper | N/A |
| Plasmid: K14-rtTA | This paper | N/A |
| Software and Algorithms | ||
| Fiji software | https://imagej.net/Fiji | |
| GSEA software | (Subramanian et al., 2005) | https://www.gsea-msigdb.org/gsea/index.jsp |
| WebGestalt | (Wang et al., 2017) | http://www.webgestalt.org/ |
| Prism 8 | GraphPad | 8.3.0 |
| Microsoft Excel | Microsoft | 2016 |
| Other | ||
| Sterile Celltrics 30 μm filters | Sysmex | Cat#: 04-004-2326 |
| Lumox dish 35 | Sarstedt | Cat#: 94.6077.331 |