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. 2021 Feb 1;67(3):490–499. doi: 10.1093/clinchem/hvaa324

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Published by Oxford University Press on behalf of the American Association for Clinical Chemistry 2021. This work is written by US Government employees and is in the public domain in the US.

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Fig. 1. — Development and validation of a reference method for the quantification of apo(a) in plasma. (A) Outline of the method development and validation. (B) Quantification strategy by targeted double isotope dilution LC-MS/MS outlining calibrators and sample preparation. (C) Sequence of the human recombinant apo(a) calibrator. Candidate quantification peptides and their locations are shown. Gray highlight, final quantification peptides; no highlight, candidate peptides not meeting quantification requirements.