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. Author manuscript; available in PMC: 2021 Aug 2.
Published in final edited form as: Oncogene. 2021 Feb 2;40(9):1690–1705. doi: 10.1038/s41388-021-01658-z

Fig 5. AR-CAMKK2-AMPK signaling increases autophagy by phosphorylating ULK1 at serine 555.

Fig 5.

(A) LNCaP cells were treated ± 10 nM R1881 (androgen) ± 30 μM STO-609 for 72 hours. (B) LNCaP-CAMKK2 cells were transfected with siRNAs targeting scramble control or the α1 catalytic subunit of AMPK (siAMPK) and then treated with androgen for 72 hours or DOX (50 ng/ml) for 48 hours. Cell lysates were subjected to immunoblot analysis. (C) Parental LNCaP cells were transfected with siRNAs targeting scramble control or three different regions of the α1 catalytic subunit of AMPK (siAMPK) and then treated with vehicle or androgen for 72 hours. Cell lysates were subjected to immunoblot analysis. (D) LNCaP-CAMKK2 cells were transfected with empty vector, ULK1 or ULK1 (4SA) expression constructs and then treated ± DOX for 48 hours. Cell lysates were subjected to immunoblot analysis. (E) Immunoblot analysis of C4-2 Cas9 control and CAMKK2 KO derivative cells treated with vehicle or chloroquine (20 μM). (F) Immunoblot analysis of 22Rv1-shCAMKK2 cells ± 800 ng/ml DOX treatment for 72 hours.