FIGURE 1.

ANO9 controlled proliferation and the cell cycle in gastric cancer (GC) cells. A, Western blotting showed that ANO9 was strongly expressed in MKN7 and MKN74 cell lines in GC. B, Western blotting revealed that ANO9 siRNA effectively reduced ANO9 protein levels in MKN7 and MKN74 cells. C, ANO9 siRNA effectively reduced ANO9 mRNA levels in MKN7 and MKN74 cells. Mean ± SEM. n = 3. *P < .05 (significantly different from control siRNA). D, Downregulation of ANO9 inhibited the proliferation of MKN7 and MKN74 cells. The number of cells was counted 48 and 72 h after siRNA transfection. Mean ± SEM. n = 3. *P < .05 (significantly different from control siRNA). E, Downregulation of ANO9 increased the number of cells in the G₂/M phase in MKN7 and MKN74 cells. Cells transfected with control or ANO9 siRNA were stained with propidium iodide (PI) and analyzed by flow cytometry. Mean ± SEM. n = 3. *P < .05 (significantly different from control siRNA)