FIGURE 4.

Binding ability to PD‐1 in ANO9‐depleted gastric cancer (GC) cells. A, Verification of gene expression by real‐time quantitative RT‐PCR. Expression levels of genes (ANO9, PD‐L1, and PD‐L2) in ANO9‐depleted MKN7 and MKN74 cells were compared with those in control siRNA‐transfected cells using real‐time quantitative RT‐PCR. Mean ± SEM. n = 3. *P < .05 (significantly different from control siRNA). B, Downregulation of ANO9 reduced binding ability to PD‐1 in MKN7 and MKN74 cells. Cells transfected with control or ANO9 siRNA and stained with recombinant PD‐1 were analyzed by flow cytometry. Mean ± SEM. n = 3. *P < .05 (significantly different from control siRNA)