FIGURE 4.

Induction of DNA double‐stranded breaks by ²¹¹At‐AAMT in PANC‐1 cells. Imaging of ²¹¹At‐AAMT genotoxicity and cytotoxicity in PANC1 or B16F10 cells. Cells were treated with 0.3‐10 kBq of ²¹¹At‐AAMT for 5 min. Even at 0.3 kBq ²¹¹At‐AAMT, cells were positive for pH2AX, and the Image‐iT^® DEAD GreenTM viability stain indicated DNA damage and compromise in plasma membrane integrity. Hoechst 33342 stain was used as a nuclear segmentation tool (A). The bar graph (B) shows the quantitative representation of ²¹¹At‐AAMT‐treated cells