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. 2021 Jan 27;112(3):1225–1234. doi: 10.1111/cas.14786

FIGURE 5.

FIGURE 5

Geldanamycin (GA) degrades ROR1 protein through the ubiquitin/proteasome pathway. Western blot analysis of ROR1, p21, and LC3B in NCI‐H1975 (A), PC‐9 (B), and HeLa cells expressing humanized fluorescent Azami‐Green‐ROR1‐intracellular domain (hAG‐ROR1‐ICD) (C), treated with proteasomal inhibitor MG132 (10 μmol/L) or lysosomal inhibitor chloroquine (10 μmol/L) for 8 h in the presence or absence of 0.5 μmol/L GA. β‐Actin served as a loading control. Columns indicate relative signal intensities of ROR1 protein. D, Immunoprecipitation (IP)‐western blot analysis using ROR1 Ab in COS‐7 cells transfected with HA‐tagged ubiquitin (HA‐Ub) and/or ROR1 in the presence or absence of 0.5 μmol/L GA for 3 h