Fig. 2. sGD elements display different super-Mendelian inheritance patterns depending on the trans-heterozygote progenitor sex.

Genetic crosses performed using the sGD transgenes in combination with vasa or nanos-driven Cas9 lines located in the first (X), second (II) or third (III) chromosomes. Graph contains data for a rab5; b spo11; c rab11; and d prosalpha2 sGDs. Single F₁ germline conversion was assessed by scoring the markers for both transgenes in the F₂ progeny. Inheritance of Cas9 and sGD is depicted using green and purple dots, respectively. Each single cross is shown as a single data point. Values for inheritance mean, number of crosses (N) and individuals scored (n) are shown atop of the graph in line with each respective dataset. Sex of the parental (F₁) trans-heterozygote is indicated in the X-axis. sGD-Cas9 combinations depicted in bold represent pairings that were progressed to cage trials and further genotypic studies. Error bars represent mean values ± SEM. Stars represent statistical significance (****p < 0.0001; ***p < 0.001; **p < 0.01; *p < 0.05) for F₁ male-to-female sGD-copying differences (black, two-sided t-test) and to detect Cas9 being inherited below Mendelian frequencies (green, χ²). Raw phenotypical data is provided as “Supplementary Data 1”.