Fig. 1. mlon-IE works at other loci and conditions in addition to its role in the fbp1 upstream region.
a Schematic representation of the fbp1 upstream region containing upstream activating sequence 1 and 2 (UAS1 and UAS2), the binding sites for Atf1 and Rst2, respectively. The numbers indicate the transcription start site of the fbp1 transcripts and the distances of UAS1, UAS2 and the TATA box from the first ATG of fbp1 open reading frame (ORF). b Representative images of northern blot showing stepwise expression of mlonRNAs and fbp1 mRNA and blot of the chromatin analysis showing stepwise chromatin remodeling during glucose starvation. Wild-type haploid cells were grown to 2.0 × 107 cells/ml in YER medium, then transferred to YED medium. Cells were harvested at the indicated times. cam1 transcript was used as an internal control. c M26 and M375 mutations in ade6 (bold italic) and inserted mlon-IE sequences are shown. d ade6 transcription in indicated cells. Diploid cells were cultured to induce meiosis, as described in the Methods section. 18S rRNA stained by ethidium bromide is shown as the loading control.