Fig. 2. Transcription from mlon-IE activates meiotic DSB formation and recombination in ade6-M26, the meiotic recombination hotspot.

a Recombination rates at the M26 or M375 control allele (indicated as ade⁺/10⁴ spores) were examined, as described in the Methods section (left). Recombination between leu1-32 and his3-D1 (indicated as leu⁺/his⁺ percentage) was measured as the control (right). Error bars represent standard deviations. n = 3 or 5 biologically independent experiments. P values were calculated using the unpaired one-sided Student’s t-test: *P < 0.05, n.s. (not significant). b Indicated haploid cells possessing the pat1-114 rad50s genotype were cultured to induce meiosis, and DNA was prepared as described in the Methods section. Meiotic DSBs were detected by Southern blotting. The dotted line indicates DSBs around M26 and inserted mlon-IE. c Meiotic chromatin remodeling in indicated cells. Diploid cells were cultured as in Fig. 1c. The black arrowhead and the dotted line indicate MNase-sensitive sites at the M26 mutation and the inserted mlon-IE, respectively.