Fig. 3. mlon-IE works downstream of binding sequences for transcription factors, CCAAT-binding factor and Rst2.

a Schematic representation of ade6-4002, ade6-4099, ade6-4095 and ade6-4156. 4002 insertion, 4099 mutation, 4095 mutation and 4156 mutation are shown (bold italic). mlon-IE was translocated 200 bp downstream from the 4002/4099/4095/4156 mutation. b ade6 transcripts of indicated cells in meiosis. Cells were cultured, as described in Fig. 1c. c Recombination rates of indicated cells were assessed, as described in Fig. 2a. Error bars represent standard deviations. n = 3 or 6 biologically independent experiments. P values were calculated using the unpaired one-sided Student’s t-test: *P < 0.05. d, e Meiotic DSBs of indicated cells were detected by Southern blotting, as described in Fig. 2b.