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. 2021 Mar 5;11:5295. doi: 10.1038/s41598-021-84651-6

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Optical SNR, amplitude (ΔF/F), and temporal summation—calcium vs. voltage imaging. (A) Image: Cortical brain slice harvested from GCaMP6f animal, with ROI and glass electrode (el.) for synaptic stimulation. Trace: Synaptically-evoked calcium transients obtained from the ROI (5 pixel spatial averaging). Synaptic stimulation comprised three pulses at 120 ms interval (8.3 Hz). Temporal average of 4 trials, bleach correction, 40 Hz low-pass. (B) Same as in (A), except brain slice harvested from a chi-VSFP animal, and much greater size of the ROI (21 pixels). (C) Amplitude (ΔF/F) of the 1st transient is represented by a raster dot plot and the color-matched bar (mean ± sem). *** indicate p < 0.00001. White (GCaMP6f): 27 recordings from 6 brain slices, in 3 GCaMP6f animals. Gray (chi-VSFP): 42 recordings from 19 brain slices, in 8 chi-VSFP animals, p < 0.00001. (D) Same as in C except, “summation ratio” was measured as an amplitude ratio between the 3rd and the 1st peak in the same optical trace.