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. 2021 Mar 5;4:292. doi: 10.1038/s42003-021-01833-8

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Citrine fluorescence in mitochondria (green) and chlorophyll autofluorescence (magenta), and bright field (BF) image of mesophyll protoplasts of MTS-Citrine transgenic A. thaliana are shown as a merged image (Merge) taken by CLSM. Scale bar: 10 µm. b Time-lapse images of mitochondria taken every 5 s. Fast, directional movements (arrowheads) and slow, wiggling movements (arrows) of mitochondria are shown. Scale bar: 2 µm. c Representative images showing the trajectories of mitochondrial movements over a 30-s period. Mitochondrial movements were tracked and quantified using Fiji software and its plugin MTrackJ. Mitochondria are labeled with numbers and outlined with different colored lines. Scale bar: 10 µm. d Speed distribution of mitochondrial movement from three independent protoplasts (protoplast No. 1, blue; protoplast No. 2, orange; and protoplast No. 3, green).