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. 2021 Mar 5;4:292. doi: 10.1038/s42003-021-01833-8

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Trajectories of mitochondrial movements constructed from time-lapse analysis of images of three different protoplasts (No. 1–3) acquired for 30 s at 250-ms intervals. Scale bar; 10 µm. b Distribution of the direct distance between the first and last point of mitochondria in the trajectories of mitochondrial movement acquired from three independent protoplasts (protoplast No. 1, blue color; protoplast No. 2, orange; and protoplast No. 3, green). c Scatter plot of speed (x-axis) and angle changes (y-axis) of mitochondria at each time point acquired from the trajectories of mitochondrial movement, which are separated based on a distance shorter (open circles) or longer (filled-magenta circles) than 5.0 µm in b. The plots are separated into two regions highlighted in gray and pale blue.