Figure 10.

OsmY-Lipase 6B expressed in BL21(DE3) and C41(DE3). Cells were cultivated in 50 mL of 2xTY auto-induction media at 37 °C using 1:100 dilution of inoculum. The cell optical densities monitored at 600 nm are shown as lines. The activity of OsmY-Lipase6B in the spent medium measured with the pNPA assay using an absorbance at 405 nm are shown as bars. The lines and bars of different samples are represented using different colours; OsmY(WT)-Lipase 6B in C41(DE3)—black, OsmY(M3)-Lipase 6B in C41(DE3)—blue, OsmY(WT)-Lipase 6B in BL21(DE3)—grey and OsmY(M3)-Lipase 6B in BL21(DE3)—orange.