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. 2021 Mar 5;11:5290. doi: 10.1038/s41598-021-84859-6

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fractional OsmY-Tfu0937 activity in the extracellular, periplasmic and cytoplasmic fractions of cells expressing Tfu0937 fused to either OsmY(WT) or OsmY(M3). Activity was measured with the pNPG assay using an absorbance at 405 nm. Experiments were performed in triplicate. Cells were cultivated in 2 × TY auto-induction medium at 37 °C for either 6 h or 24 h. The enzyme activity in each fraction was calculated as a percentage of total activity in all three fractions.