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. 2021 Mar 5;11:5290. doi: 10.1038/s41598-021-84859-6

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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OsmY-Tf0937 secretion using C41(DE3) and BL21(DE3) in 400-mL 2 × TY auto-induction media, at 37 °C and 200 rpm. Cultures were inoculated using 1:100 dilution and samples were collected at 7 h and 24 h of cultivation. (a) SDS-PAGE of spent medium. Lane L: protein marker, Lane 1 and 5: OsmY(WT)-Tfu0937 in BL21(DE3), Lane 2 and 6: OsmY(M3)-Tfu0937 in BL21(DE3), Lane 3 and 7: OsmY(WT)-Tfu0937 in C41(DE3), Lane 4 and 8: OsmY(M3)-Tfu0937 in C41(DE3). (b) pNPG assay of the spent medium.