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. 2021 Mar 5;11:5290. doi: 10.1038/s41598-021-84859-6

Figure 9.

Figure 9

OsmY-RFP expression in E. coli BL21(DE3). Cells were cultivated in 20 mL of 2xTY auto-induction media at 37 °C using 1:100 dilution of inoculum. The supernatant and cells from 20 µL culture were used for fluorescence measurement using Ex = 584 nm and Em = 607 nm. (a) Growth curve of the culture. (b) Fluorescence intensity of the spent medium. (c) Fluorescence intensity of the cells. The lines and bars of different variants are represented using different colours; OsmY(WT)-RFP—grey and OsmY(M3)-RFP—orange.