. 2021 Mar 5;11:5290. doi: 10.1038/s41598-021-84859-6

Table 2.

Production of secretory protein using OsmY(WT) and OsmY(M3) for three different target proteins.

Target protein	M_w (kDa)	Theoretical pI	Protein source organism	Total secretory protein production by OsmY(M3) relative to OsmY(WT)/cultivation time (h)	Recombinant protein expression host
Tfu0937	53.4	4.81	Thermobifida fusca YX	3.1 ± 0.3/25 2.9 ± 0.8/24	BL21(DE3) C41(DE3)
RFP	25.4	5.65	Synthetic construct	1.8 ± 0.6/24	BL21(DE3)
Lipase 6B	19.7	6.96	Bacillus subtilis 168	2.5 ± 0.3/10	C41(DE3)

Target protein

M_w (kDa)

Theoretical pI

Protein source organism

Total secretory protein production by OsmY(M3) relative to OsmY(WT)/cultivation time (h)

Recombinant protein expression host

Tfu0937

53.4

4.81

Thermobifida fusca YX

3.1 ± 0.3/25

2.9 ± 0.8/24

BL21(DE3)

C41(DE3)

RFP

25.4

5.65

Synthetic construct

1.8 ± 0.6/24

BL21(DE3)

Lipase 6B

19.7

6.96

Bacillus subtilis 168

2.5 ± 0.3/10

C41(DE3)