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. 2021 Mar 5;10(3):24. doi: 10.1038/s41389-021-00315-0

Fig. 3. RNF8 promotes RecQL4 dissociation from DSBs.

Fig. 3

A Knockdown of RNF8 significantly inhibits the dissociation of RecQL4 from DSBs. U2OS cells with or without RNF8 depletion were treated with 365-nm micro-point laser. The recruitment of GFP-RecQL4 at DSBs was recorded and the fluorescence density was quantified. B The prolonged and enhanced recruitment of RecQL4-3M at DSBs induced by UV micro-point laser. U2OS cells were transfected with GFP-tagged WT RecQL4 or its 3M mutant, and subjected to UV micro-point laser treatment. The recruitment of GFP-RecQL4 or its mutant at DSBs were recorded and the fluorescence density at DSBs was quantified. At least 15 cells were analyzed for each treatment. The data represent mean ± SEM from three independent experiments. **p < 0.01.