TABLE 2.
Genotyping primers
| Name | Purpose/Annealing temperature | Sequence [5’−3’] | Amplicon size [bp] |
|---|---|---|---|
| Ppp1r3c-WT-F Ppp1r3c-WT-R |
Detection of Ppp1r3c WT/57°C | GAGCTGTGTCAGACTTGTTCAGATAGAGC TTGAAAACCATTGTAAGGACCCAGGAAACTC |
400 |
| Ppp1r3c-KO-F Ppp1r3c-KO-R |
Detection of Ppp1r3c WT/55°C | AGATCTCATCACCCCAGTGC TAGTTCCCAGGCTGTCCTTG |
192 |
| Ppp1r3d-WT-F Ppp1r3d-WT-R |
Detection of Ppp1r3d WT/55°C | CGAAGATGTCCTCGGTACCTTCAGC CAGGATCTGGAGTTCACGCTGC |
274 |
| Ppp1r3d-KO-F Ppp1r3d-KO-R |
Detection of Ppp1r3d KO/57°C | GCAGCCTCTGTTCCACATACACTTCA AGGCCTTTCTTGCAAAACACTGACC |
438 |
| Epm2a-F Epm2a-KO-R Epm2a-WT-R |
Detection of Epm2a WT and KO/55°C Detection of Epm2a KO/55°C Detection of Epm2a WT/55°C |
GCATCGGCTGTAAGTTAGCC AGCGTATTCAATAACCCTTAAT CGTGTGTCCATTCTCCAGAA |
430 (KO) 620 (WT) |