Fig. 3.

Tight junction-forming capability of Cld5 is required for controlling paracellular permeability of cultured endothelial cells. SVECs were retrovirally transfected with control vector (Cont), Cldn5-ligated vector (Cld5) or Clnd5 F147A mutant-ligated vector (Cld5 F147A). Stable clones were established and examined by immunofluorescence (A) and western blotting (B) analyses with anti-Cld5 antibody. (C) TER of confluent SVECs stably expressing one of the above vectors was measured 5 days after cell seeding in trans-wells. Student’s t-test was performed between Cont and Cld5, and Cont and Cld5F147A. **P < 0.01; N.S., not significant.