Developmental expression of the avian β-defensin 10 mRNA in the chicken yolk sac tissue. Avian β-defensin 10 (AvBD10) mRNA was detected in formalin-fixed, paraffin embedded yolk sac tissue at embryonic day 7 (E7), 11 (E11), 15 (E15), and 19 (E19) by RNAscope in situ hybridization and revealed as brown dots (Wang et al., 2012). Tissues were counterstained with hematoxylin. The scale bar represents 0.1 mm (Zhang and Wong, 2019).