Table 3.

RT-PCR primers and GenBank accession numbers of chicken.

Target	Primer sequence (5′–3′)a	Accession no.	Product size, bp
IL-1β	F:ACTGGGCATCAAGGGCTA R:GGTAGAAGATGAAGCGGGTC	NM_204524	131
TNF-α	F: GAGCGTTGACTTGGCTGTC R: AAGCAACAACCAGCTATGCAC	NM_204267	64
IL-8	F: ATGAACGGCAAGCTTGGAGCTG R:TCCAAGCACACCTCTCTTCCATCC	AJ_009800	103
IFN-γ	F: AGCTGACGGTGGACCTATTATT R:GGCTTTGCGCTGGATTC	Y07922	259
GAPDH	F:TGCTGCCCCAGAACATCATCC R: ACGGCAGGTCAGGTCAACAA	NM_204305.1	108

Abbreviations: RT-PCR, reverse-transcription polymerase chain reaction; IL-1β, interleukin-1β; TNF-α, tumor necrosis factor; IL-8, interleukin-8; IFN-γ interferon-γ; GAPDH, reduced glyceraldehyde phosphate dehydrogenase.