Effect of PFA supplementation on circulating antioxidant markers in HS broilers. The relative gene expression of SOD1 (A), SOD2 (B), and GPX-1 (C) was determined by qPCR and analyzed by 2–ΔΔCt method using the C-TN group as a calibrator. Main effects of diet, temperature, and the interaction are presented next to each graph. Data are presented as mean ± SEM (n = 6–8 birds/group). Different letters indicate significant difference at P < 0.05. Abbreviations: CCL, C motif chemokine ligands; CXCL, C-X-C motif chemokine ligand; GPX, glutathione peroxidase; HS, heat stress; PFA, phytogenic feed additive; SOD, superoxide dismutase; TN, thermoneutral; TN, theroneutral.