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. 2020 Nov 5;100(3):100801. doi: 10.1016/j.psj.2020.10.055

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© 2020 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Effect of PFA supplementation on circulating heat shock proteins and heat shock factors in HS broilers. The relative gene expression of HSP27 (A), HSP60 (B), HSP70 (C), HSP90 (D), HSF1 (E), HSF2 (F), HSF3 (G), and HSF4 (H) was determined by qPCR and analyzed by 2^–ΔΔCt method using C-TN group as a calibrator. Main effects of diet, temperature, and the interaction are presented next to each graph. Data are presented as mean ± SEM (n = 6–8 birds/group). Different letters indicate significant difference at P < 0.05. Abbreviations: HS, heat stress; PFA, phytogenic feed additive.