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. 2020 Nov 30;100(3):100869. doi: 10.1016/j.psj.2020.11.046

Figure 5.

Figure 5

CLC and LPHFCLC induced hepatic steatosis in Plymouth Rock chickens. (A) Body weights of Plymouth Rock chickens fed CON, LPHF, CLC, or LPHFCLC at weeks 0, 2, and 4. (B) Appearance of livers in Plymouth Rock chickens fed the above indicated diets for 4 wk. (C) Oil Red O staining was used to detect the severity of lipid accumulation in livers of Plymouth Rock chickens fed the indicated diets for 4 wk. The amount of (D) TG and (E) cholesterol in the livers of Plymouth Rock chickens fed with the indicated diets for 4 wk. The values for hepatic TG and cholesterol were normalized with the weights of liver samples. CON, n = 6; LPHF, n = 5, CLC, n = 8; LPHFCLC, n = 6. Data are shown as mean ± SEM. Groups with no significant difference were labeled with a common letter (1-way ANOVA). Abbreviations: CLC, high cholesterol and low choline diet; CON, control diet; LPHF, low protein and high fat diet; LPHFCLC, low protein, high fat, high cholesterol, and low choline diet; TG, triglyceride.