Figure 2.

Bacteria composition at the (A) phylum and (B) genus levels of broiler chickens with treatments groups 1—control (CTRL), 2—in-feed antibiotic treatment containing CTRL + 0.05% bacitracin methylene disalicylate, 3—CTRL diet + in-water probiotic (containing 0.025 g/L of Bacillus subtilis fermentation extract), 4—in-feed probiotic containing CTRL + 0.005% B. subtilis, 5—in ovo saline group injected with 200 μL of physiological saline (0.9% NaCl), and 6—in ovo probiotic group injected with 200 μL of B. subtilis fermentation extract (10 × 10⁶ cfu). The cecal content was collected from 28-day-old chickens. DNA was extracted from the cecal content, and relative abundances are shown as determined by Illumina sequencing and visualized with the web-based tool MicrobiomeAnalyst.