Table 5.
Effect of Bacillus subtilis fermentation extract delivery route on cecal short-chain fatty acid concentrations in broiler chickens raised for 28 d.
| Short-chain fatty acid concentration (μM) | Treatments1 |
SEM | P value2 | |||||
|---|---|---|---|---|---|---|---|---|
| Control | In-feed antibiotics | In-water probiotic | In-feed probiotic | In ovo saline | In ovo probiotic | |||
| Acetic acid | 47.4 | 55.0 | 43.5 | 48.7 | 49.5 | 52.7 | 2.44 | 0.832 |
| Propionic acid | 1.98 | 1.69 | 3.16 | 2.57 | 2.10 | 2.73 | 0.24 | 0.535 |
| Butyric acid | 13.7 | 12.4 | 11.5 | 7.15 | 10.1 | 13.4 | 0.82 | 0.184 |
| Valeric acid | 0.28 | 0.16 | 0.44 | 0.12 | 0.19 | 0.30 | 0.04 | 0.107 |
| Lactic acid | 2.05 | 3.23 | 4.93 | 2.52 | 2.13 | 4.66 | 0.63 | 0.664 |
| Branched-chain fatty acids | 0.40 | 0.07 | 0.30 | 0.33 | 0.16 | 0.22 | 0.04 | 0.321 |
| Volatile fatty acids | 63.8 | 69.4 | 58.9 | 58.9 | 62.0 | 69.4 | 3.05 | 0.865 |
| Total short-chain fatty acids | 65.8 | 72.6 | 63.8 | 61.4 | 64.2 | 74.1 | 2.92 | 0.790 |
1
Treatment groups include control (CTRL), in-feed antibiotics treatment containing CTRL + 0.05% bacitracin methylene disalicylate (BMD), CTRL diet + in-water probiotic (containing 0.025 g/L of Bacillus subtilis fermentation extract), in-feed probiotic containing CTRL + 0.005% Bacillus subtilis, in ovo saline group injected with 200 μL of physiological saline (0.9% NaCl), and in ovo probiotic group injected with 200 μL of Bacillus subtilis fermentation extract (10 × 106 cfu) in n = 6 replicates per treatment.
2
Significance was set at P ≤ 0.05.