Table 1.
Primers for real time qPCR.
| Gene | Sequence (5′-3′) | Accession number | Tm (°C)1 |
|---|---|---|---|
| HSP70 | F: GGTAAGCACAAGCGTGACAATGCT | NM_001006685.1 | 55°C |
| R: TCAATCTCAATGCTGGCTTGCGTG | |||
| HSP60 | F: TGTGTGGAGCAGCAAGACAGAGA | NM_001012916.1 | 55°C |
| R: TTCATGAGCTCCCAATCCCAGACA | |||
| HSP40 | F: GGGCATTCAACAGCATAGA | NM_001199325.1 | 60°C |
| R: TTCACATCCCCAAGTTTAGG | |||
| HSF1 | F: AAGTCACCAGCGTGTCCAG | NM_001305256.1 | 60°C |
| R: GCCTCGTTCTCATGCTTCA | |||
| HSF2 | F: TACTGCATTTCCGCTGCTC | NM_001167764.2 | 60°C |
| R: AGGGGTTTGTCCACAGAGG | |||
| HSF3 | F: ACGACGTCATCTGCTGGAG | NM_001305041.1 | 60°C |
| R: TTGAGCTGTCGGATGAAGC | |||
| GAPDH | F: AGAACATCATCCCAGCGT | NM_204305.1 | 60°C |
| R: AGCCTTCACTACCCTCTTG |
Abbreviations: HSP, heat shock proteins; HSF, heat shock factor; GAPDH, Glyceraldehyde-3-phosphate dehydrogenase.
1
The Tm value was referred to the annealing temperatures of each genes for RT-qPCR analyzed.