Table 1.

Sequence of primers used in quantitative real-time PCR.

Gene	Reference sequence	Forward primer sequences (5'–3')	Reverse primer sequences (5'–3')
β-actin	NM 205,518.1	CCGCTCTATGAAGGCTACGC	CTCTCGGCTGTGGTGGTGAA
iNOS	NM 204,961.1	CCTGGAGGTCCTGGAAGAGT	CCTGGGTTTCAGAAGTGGC
NF-κB	NM 205,134.1	TCAACGCAGGACCTAAAGACAT	GCAGATAGCCAAGTTCAGGATG
COX-2	NM 0.011,67718.1	TGTCCTTTCACTGCTTTCCAT	TTCCATTGCTGTGTTTGAGGT
PTGES	NM 0.011,94983.1	GTTCCTGTCATTCGCCTTCTAC	CGCATCCTCTGGGTTAGCA
TNF-α	NM 204,267.1	GCCCTTCCTGTAACCAGATG	ACACGACAGCCAAGTCAACG
Interferon gamma	NM 205,149.1	GCTGACGGTGGACCTATTATTGTAGAG	TTCTTCACGCCATCAGGAAGGTTG
IL-2	NM 204153.1	CTGTATTTCGGTAGCAATG	ACTCCTGGGTCTCAGTTG
IL-6	NM 204628.1	AAATCCCTCCTCGCCAATCT	CCCTCACGGTCTTCTCCATAAA
IL-1β	NM 204524.1	ACTGGGCATCAAGGGCTACA	GCTGTCCAGGCGGTAGAAGA
Small HSP	NM 001010842.2	CGGCTTCATCTCCAGGTGCTTC	TTGGCTGGTTCTTCCTTCTTGGC
HSP27	NM 205290.1	ACACGAGGAGAAACAGGATGAG	ACTGGATGGCTGGCTTGG
HSP40	NM 001199325.1	GGGCATTCAACAGCATAGA	TTCACATCCCCAAGTTTAGG
HSP60	NM 001012916.1	AGCCAAAGGGCAGAAATG	TACAGCAACAACCTGAAGACC
HSP70	NM 001006685.1	CGGGCAAGTTTGACCTAA	TTGGCTCCCACCCTATCTCT
HSP90	NM 001109785.1	TCCTGTCCTGGCTTTAGTTT	AGGTGGCATCTCCTCGGT
HSP110	NM 001159698.1	GGCTCCAGTATTACGCTGCGATAG	GCACTCACAGCATTGTTCATCCAC
HSF1	NM 001305256.1	GTCGCAGGAGCAGCAGAAGC	CTGAGCCGTGTAGTGAACCAACTG
HSF2	NM 001006685.1	TTCCGCTGCTCGCATTCCTTG	GCCTCACTTGCTTCTGCCTCTG
HSF3	NM 001305041.1	AGGCAGAAGCACAGTCAACAACAG	GACGGCTGTACTTGGAAGGTGAAG
HSF4	NM 001172374.1 84	TGCCAGCCTTCCTAACCAAG	TGGTGCCATTCGTACTCCAG
HSP10	NM 205067.1	GCGCAGCAGAGACCGTAACC	TCCAACTGCTACCACTGTTGCTTG

Abbreviations: COX-2, cyclooxygenase-2; HSF, heat shock factor; HSP, heat shock protein; iNOS, inducible nitric oxide synthase; NF-κB, nuclear transcription factor-kappa B; PTGES, prostaglandin E synthase; TNF-α, tumor necrosis factor alpha.