Figure 2.

The mRNA expression of zonula occludens-1 (ZO-1) (A), claudin-1 (B), claudin-3 (C), and occludin (D) in the jejunum of broilers fed diets supplementation with xylo-oligosaccharides (XOS), ⁶⁰Co γ-ray–irradiated Astragalus polysaccharides (IAPS), and their combination on day 21. The data are represented as the mean value ± SEM of each treatment (n = 6). Means without a common letter significantly differ (P ≤ 0.05). ^a–cSuperscripts for means belong to 1-way ANOVA among 5 treatment groups; P values means the significant from 2 × 2 factorial analysis (excluded the AGP group), and the model included the main effects of XOS and IAPS supplementation as well as XOS × IAPS interaction. Abbreviations: Control, basal diet; AGP, basal diet supplemented with 50 mg/kg chlortetracycline; IAPS, basal diet supplemented with 600 mg/kg IAPS; XOS, basal diet supplemented with 100 mg/kg XOS; XOS + IAPS, basal diet supplemented with 100 mg/kg XOS and 600 mg/kg IAPS.