Table 2.

Primer sequences used for RT-qPCR analysis.

Genes	Primer sequences (5′-3′)	Amplicon sizes (bp)	GenBank number
Claudin-1	F: GACCAGGTGAAGAAGATGCGGATG	107	NM 001013611.2
	R: CGAGCCACTCTGTTGCCATACC
Claudin-3	F: CTTCATCGGCAACAACATCGTGAC	113	NM 204202.1
	R: CCAGCATGGAGTCGTACACCTTG
ZO-1	F: CTTCAGGTGTTTCTCTTCCTCCTC	131	XM 015278981.2
	R: CTGTGGTTTCATGGCTGGATC
Occludin	F: TCATCGCCTCCATCGTCTAC	240	NM 205128.1
	R: TCTTACTGCGCGTCTTCTGG
β-actin	F: ATCCGGACCCTCCATTGTC	120	NM 205518.1
	R: AGCCATGCCAATCTCGTCTT

Abbreviations: RT-qPCR, real-time quantitative PCR; ZO-1, zonula occludens-1.