. 2020 Dec 29;100(3):100967. doi: 10.1016/j.psj.2020.12.064

Table 5.

Effects of hulless barley and β-glucanase on ileal short-chain fatty acids of broiler chickens aged 28 d.

HB¹ (%)	BGase (%)	SCFA μmol/g of wet ileal content									Molar percentage of total SCFA
HB¹ (%)	BGase (%)	Total	Ace	Pro	Buty	Isob	Val	Isov	Cap	Lac	Ace	Pro	Buty	Isob	Val	Isov	Cap	Lac
0	0	175.3	65.2	24.4	11.2	2.3^a,b	3.4	3.1	1.5	63.8	37.2	13.9	6.4	1.3^a,b	1.9	1.7	0.8	36.3
	0.01	172.9	64.9	24.1	11.2	2.2^a,b	2.9	3.0	1.4	62.9	37.5	13.9	6.4	1.2^a,b	1.7	1.7	0.8	36.3
	0.1	164.5	62.1	23.2	10.3	1.8^a,b	2.2	2.4	1.0	61.0	37.9	14.1	6.3	1.0^a,b	1.3	1.4	0.6	37.0
30	0	160.3	61.9	22.1	10.0	1.9^a,b	2.6	2.5	1.0	58.0	38.7	13.4	6.4	1.2^a,b	1.6	1.5	0.6	36.2
	0.01	167.5	63.2	22.6	10.7	2.8^a,b	2.6	2.5	1.1	61.8	37.9	13.3	6.3	1.5^a,b	1.5	1.5	0.6	37.0
	0.1	171.1	65.4	23.3	10.9	1.6^a,b	2.4	2.7	1.2	63.2	38.2	13.4	6.4	0.9^a,b	1.4	1.5	0.7	37.2
60	0	165.9	61.8	22.3	10.5	2.7^a,b	3.3	2.9	1.3	60.6	37.5	13.1	6.4	1.6^a,b	1.9	1.7	0.7	36.6
	0.01	182.5	70.4	25.8	11.3	1.1^b	2.8	2.8	1.3	66.6	38.5	14.1	6.2	0.7^b	1.5	1.5	0.7	36.4
	0.1	157.2	59.1	20.8	10.3	2.9^a	2.6	2.2	1.0	58.0	37.6	13.3	6.5	1.8^a	1.6	1.4	0.6	36.9
SEM²		3.15	1.17	0.51	0.24	0.13	0.12	0.12	0.05	1.16	0.14	0.16	0.08	0.07	0.06	0.06	0.02	0.15
Main effects
HB (%)
0		170.9	64.1	23.9	10.9	2.1	2.9	2.8	1.3	62.6	37.5	14.0	6.4	1.2	1.6	1.6	0.7	36.5
30		166.3	63.5	22.7	10.5	2.1	2.5	2.6	1.1	61.0	38.3	13.3	6.4	1.2	1.5	1.5	0.6	36.8
60		168.5	63.8	23.0	10.7	2.3	2.9	2.7	1.2	61.7	37.9	13.5	6.4	1.4	1.7	1.5	0.7	36.6
BGase (%)
0		167.2	63.0	22.9	10.6	2.3	3.1	2.8	1.3	60.8	37.8	13.5	6.4	1.4	1.8^a	1.7	0.7	36.4
0.01		174.3	66.2	24.2	11.0	2.0	2.8	2.8	1.3	63.7	38.0	13.8	6.3	1.1	1.6^a,b	1.6	0.7	36.6
0.1		164.2	62.2	22.5	10.5	2.1	2.4	2.4	1.1	60.7	37.9	13.6	6.4	1.2	1.4^b	1.4	0.6	37.0
ANOVA P-value
HB		0.823	0.980	0.555	0.796	0.858	0.406	0.630	0.431	0.837	0.146	0.240	0.978	0.546	0.471	0.814	0.510	0.793
BGase		0.375	0.318	0.346	0.625	0.615	0.073	0.331	0.174	0.438	0.862	0.795	0.921	0.404	0.050	0.350	0.137	0.249
HB × BGase		0.462	0.301	0.349	0.759	0.003	0.702	0.700	0.281	0.456	0.241	0.786	0.956	0.001	0.599	0.870	0.423	0.887

^a-dMeans within a main effect or interaction not sharing a common superscript are significantly different (P ≤ 0.05).

HB, hulless barley; Bgase, β-glucanase; SCFA, short-chain fatty acids; Ace, acetic acid; Pro, propionic acid; Buty, butyric acid; Isob, isobutyric acid; Val, valeric acid; Isov, isovaleric acid; Cap, caproic acid; Lac, lactic acid.

SEM = pooled standard error of mean (n = 20 birds per treatment).