Table 8.

Effects of hulless barley and β-glucanase on gastrointestinal tissue weights and lengths (proportional to body weight) of broiler chickens aged 28 d.

HB1 (%)	BGase (%)	Empty weight								Length
		Crop	Proven	Gizzard	Duo	Jejunum	Ileum	SI	Ceca	Colon	Duo	Jejunum	Ileum	SI	Ceca	Colon
0	0	0.28b	0.40	1.14c,d	0.75a,b	1.40	0.91	3.05a,b	0.35	0.13c,d	1.67b	4.09	3.89	9.95a,b	1.58	0.38b
	0.01	0.28b	0.40	1.09c,d	0.72a,b	1.29	0.84	2.84a,b	0.33	0.12d	1.66b	3.70	3.58	8.93c	1.55	0.34b
	0.1	0.30a,b	0.37	1.06d	0.74a,b	1.37	0.91	3.01a,b	0.37	0.13c,d	1.69b	3.92	3.79	9.39b,c	1.59	0.38b
30	0	0.31a,b	0.38	1.24a,b,c	0.73a,b	1.28	0.93	2.94a,b	0.36	0.14c,d	1.74a,b	4.07	3.98	9.78a,b,c	1.66	0.41a,b
	0.01	0.28b	0.38	1.19b,c,d	0.71b	1.29	0.90	2.89a,b	0.37	0.13c,d	1.74a,b	4.03	4.01	9.76a,b,c	1.69	0.37b
	0.1	0.28b	0.38	1.24a,b,c	0.73a,b	1.25	0.85	2.83b	0.39	0.14c,d	1.81a,b	3.93	3.96	9.70a,b,c	1.69	0.40a,b
60	0	0.34a	0.38	1.19b,c,d	0.72a,b	1.37	1.00	3.03a,b	0.36	0.17a,b	1.72a,b	4.21	4.18	10.25a,b	1.67	0.41a,b
	0.01	0.28b	0.40	1.38a	0.80a	1.35	0.98	3.13a	0.39	0.18a	1.95a	4.39	4.32	10.64a	1.83	0.45a
	0.1	0.29a,b	0.38	1.32a,b	0.73a,b	1.30	0.91	2.94a,b	0.37	0.15b,c	1.75a,b	4.01	4.11	10.07a,b	1.69	0.39a,b
SEM2		0.003	0.005	0.015	0.007	0.012	0.010	0.019	0.006	0.002	0.017	0.042	0.041	0.077	0.017	0.005
Main effects
HB (%)
0		0.29	0.39	1.10	0.74	1.35a	0.89b	2.97	0.35	0.13	1.67	3.90b	3.76c	9.42	1.57b	0.37
30		0.29	0.38	1.22	0.72	1.28b	0.89b	2.89	0.37	0.14	1.76	4.01a,b	3.98b	9.75	1.68a	0.39
60		0.31	0.39	1.30	0.75	1.34a,b	0.97a	3.03	0.37	0.16	1.81	4.21a	4.21a	10.32	1.73a	0.42
BGase (%)
0		0.31	0.39	1.19	0.73	1.35	0.95	3.01	0.36	0.15	1.71	4.13	4.02	9.99	1.64	0.40
0.01		0.29	0.39	1.22	0.74	1.31	0.91	2.95	0.37	0.14	1.78	4.04	3.97	9.78	1.69	0.39
0.1		0.29	0.38	1.20	0.73	1.31	0.89	2.93	0.38	0.14	1.75	3.95	3.95	9.72	1.66	0.39
ANOVA P-value
HB		0.159	0.824	0.001	0.227	0.013	0.002	0.099	0.166	0.001	0.004	0.009	0.001	<0.001	0.005	0.003
BGase		0.005	0.565	0.523	0.857	0.217	0.069	0.236	0.400	0.117	0.238	0.212	0.774	0.262	0.491	0.629
HB × BGase		0.038	0.883	0.009	0.043	0.206	0.193	0.040	0.431	0.002	0.032	0.099	0.262	0.020	0.272	0.019

^a-dMeans within a main effect or interaction not sharing a common superscript are significantly different (P ≤ 0.05).

¹HB, hulless barley; Bgase, β-glucanase; Proven, proventriculus; Duo, duodenum; SI, small intestine.

²SEM = pooled standard error of mean (n = 20 birds per treatment).