Fig. 2.
Fluorescence microscopy images of S. aureus based on PI staining.
A–E. Bacterial cells were treated with HB photoactivated by 460 nm LED light, prior to PI staining, and representative fluorescent images were shown. (A) 0 nM, 0 J cm‐2; (B) 500 nM, 0 J cm‐2; (C) 500 nM, 1 J cm‐2; (D) 500 nM, 5 J cm‐2; (E) 500 nM, 9 J cm‐2. The graphs were magnified 200 times under microscopy. Scale bars correspond to 10 µm.
E. Fluorescence intensities per image for control or treated bacteria, as calculated by ImageJ software. At least 6 separate images for each group were used, and the derived data were normalized against the ‘500 nM, 1 J cm‐2’ group.
F. Relative mRNA levels of LrgA, LrgB and LyrR in S. aureus incubated with 500 nM HB exposed by 1 J cm‐2 of light. The expression data of each gene were normalized against the respective dark control. Values represent averages of at least triplicate data, and error bars indicate the standard deviation. ***P < 0.001, *P < 0.05.