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. 2021 Feb 22;118(9):e2025343118. doi: 10.1073/pnas.2025343118

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Fig. 5. — Faster PtdIns(4,5)P₂ recovery in L_o domains than in L_d after receptor activation. (A) Time courses of averaged heterologous nFRET_eff between CFP-PH_PLCδ1 and L10-YFP (red) or S15-YFP (blue) during and after 20-s stimulation of the M₁ receptor by Oxo-M application (L10: n = 8 cells and S15: n = 14 cells). Error bars show SEM. The absolute resting FRET_eff used for normalizations was L10: 20.2 ± 2.8% and S15: 20.5 ± 1.5%. (B) PtdIns(4,5)P₂ dynamics during and after 60-s Oxo-M application (L10: n = 15 cells and S15: n = 15 cells). The absolute resting FRET_eff used for normalizations was L10: 20.1 ± 2.7% and S15: 21.3 ± 5.2%. (C) PtdIns(4,5)P₂ dynamics when 5 mM of MβCD was applied immediately following 60 s of Oxo-M (L10: n = 7 cells and S15: n = 9). The absolute resting FRET_eff used for normalizations was L10: 22.4 ± 3.3% and S15: 24.3 ± 4.4%. (D) Quantification of the lipid recovery time constants for different conditions. *P < 0.05, ***P < 0.001.