Table 1.
Identification, BPI, VIP values and P‐values of GAs under the two culture methods of Ganoderma lucidum.
NO. | Ret. Time | [M‐H]‐ m/z | UV (254 nm) | BPI | VIP pred | P‐value | Identification | Type | |||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
SC‐4 | SC‐6 | SC‐8 | LSSC‐4 | LSSC‐6 | LSSC‐8 | ||||||||
1 | 14.25 | 629.2894 | + | 43.88 | 2.17 | 31.41 | 322.88 | 197.76 | 182.01 | 5.87 | 1.08E‐02 | Ganoderic acid Mb/Mc a | I |
2 | 15.47 | 571.2916 | + | 2.53 | 0.00 | 0.00 | 173.47 | 180.28 | 157.52 | 5.52 | 1.53E‐05 | Ganoderic acid Ma/W a | I |
3 | 14.71 | 643.3068 | + | 2.74 | 0.00 | 1.09 | 185.25 | 148.72 | 122.02 | 5.14 | 1.20E‐03 | 7‐O‐methyl ganoderic acid O | I |
4 | 14.93 | 569.2788 | + | 0.00 | 0.00 | 0.00 | 125.27 | 157.62 | 148.38 | 5.07 | 1.17E‐04 | Ganoderic acid Q | II |
5 | 14.41 | 587.2913 | + | 1.22 | 0.00 | 0.00 | 141.82 | 128.48 | 123.70 | 4.85 | 1.76E‐05 | Ganoderic acid Mh | I |
6 | 15.65 | 571.3004 | + | 0.00 | 0.00 | 0.00 | 94.93 | 133.16 | 125.56 | 4.56 | 5.43E‐04 | Ganoderic acid Ma/W a | I |
7 | 14.47 | 629.3060 | + | 0.00 | 0.00 | 1.00 | 103.77 | 120.29 | 115.16 | 4.50 | 2.10E‐05 | Ganoderic acid Mb/Mc a | I |
8 | 15.30 | 611.2816 | + | 15.91 | 28.11 | 4.46 | 250.62 | 193.31 | 169.07 | 4.26 | 1.70E‐03 | Ganoderic acid T | II |
9 | 15.97 | 585.3181 | + | 0.00 | 0.00 | 0.00 | 75.90 | 103.45 | 89.40 | 3.99 | 3.54E‐04 | Ganoderic acid Md | I |
10 | 16.50 | 553.2908 | + | 0.00 | 14.87 | 0.00 | 68.01 | 110.97 | 114.65 | 3.98 | 4.15E‐03 | Ganoderic acid R | II |
11 | 14.58 | 527.2855 | + | 0.00 | 0.00 | 0.00 | 53.15 | 85.30 | 72.61 | 3.50 | 1.67E‐03 | Ganoderic acid V | I |
12 | 12.44 | 543.2775 | + | 0.00 | 0.00 | 0.00 | 36.84 | 62.04 | 51.93 | 2.95 | 2.36E‐03 | Ganoderic acid Mi | I |
13 | 14.59 | 601.3106 | + | 0.00 | 0.00 | 0.00 | 86.85 | 66.85 | 22.44 | 2.60 | 1.10E‐02 | Ganoderic acid Mg | I |
BPI, base peak intensity; VIP, variable importance for the projection.
The two ganoderic acids were indistinguishable, but it did not affect the analysis of the result.