Table 1.
Strains/plasmids | Relevant characteristic | Sources |
---|---|---|
Strains | ||
E. coli | ||
DH5α | f80dlacZΔM15, Δ(lacZYA‐argF)U169, deoR, recA1, endA1, hsdR17(rk−, mk+), phoA, supE44, l−, thi‐1, gyrA96, relA1 | Transgen |
BL21(DE3) | ompT gal dcm lon hsdSB(rB − mB−) l (DE3 [lacI lacUV5‐T7 gene 1 ind1 sam7 nin5]) | Transgen |
C. thermocellum | ||
DSM1313 | Wild type strain | DSMZ |
DSM1313::pHK‐LCC | Derived from DSM1313, with the plasmid pHK‐LCC | This work |
Plasmids | ||
pHK | pNW33N derivative, E. coli‐C. thermocellum shuttle vector, CmR/TmR | Zhang et al. (2017) |
pHK‐LCC | pHK derivative for expression of LCC in DSM1313 | This work |