Table 1.
Summary of Tm values for each of the organisms used in the HRM assay.
| Organisms | Tm of target 23S rRNA | Tm of target cryptic plasmid | ||
|---|---|---|---|---|
| Range | Mean ± SD | Range | Mean ± SD | |
| C. trachomatis (WT, n = 28) | 78.40–78.48 | 78.43 ± 0.03 | 81.14–81.16 | 81.14 ± 0.01 |
| Plasmid (C1514T mutation, n = 1) | 78.00–78.06 | 78.02 ± 0.02 | 81.19–81.25 | 81.21 ± 0.02 |
| Plasmid (C1515T mutation, n = 1) | 77.84–77.94 | 77.90 ± 0.04 | 81.17–81.25 | 81.22 ± 0.04 |
| Plasmid (C1523A mutation, n = 1) | 77.84–77.86 | 77.85 ± 0.01 | 81.17–81.20 | 81.19 ± 0.01 |
| Herpes simplex virus types 1 (n = 2) | NA | NA | NA | NA |
| Herpes simplex virus types 2 (n = 2) | NA | NA | NA | NA |
| Neisseria gonorrhoeae (n = 2) | NA | NA | NA | NA |
| Chlamydia pneumonia (n = 2) | 77.69–77.70 | 77.69 ± 0.01 | NA | NA |
| Treponema pallidum (n = 2) | NA | NA | NA | NA |
| Trichomonas vaginalis (n = 2) | NA | NA | NA | NA |
| Mycoplasma hominis (n = 2) | NA | NA | NA | NA |
| Ureaplasma urealyticum (n = 2) | NA | NA | NA | NA |
| Mycoplasma genitalium (n = 2) | NA | NA | NA | NA |
| Ureaplasma parvum (n = 2) | NA | NA | NA | NA |
NA, no amplification was observed; WT, wild‐type.