Table 2.

Limit of detection of each target gene using regression probit analysis.

Target	No. of replicates detected at each dilution/total no. of replicated at indicated no. of copies/reaction (% positive rate)							Final LOD, no. of copies/reaction
	200	100	50	20	10	5	1
23S rRNA	12/12 (100)	12/12 (100)	12/12 (100)	12/12 (100)	10/12 (83.3)	7/12 (58.3)	0/12 (0)	11.26 (95% CI, 8.80–19.87)
Cryptic plasmid	12/12 (100)	12/12 (100)	12/12 (100)	12/12 (100)	11/12 (91.7)	8/12 (66.7)	0/12 (0)	9.46 (95% CI, 7.58–14.57)