Fig. 1.

Glucagon receptor inhibition or genetic ablation protects against caspase-8–mediated apoptosis. Blood glucose (A) and C-peptide (B) concentrations were measured from plasma 10 d after inducing apoptosis. Immunofluorescent imaging of insulin (green) and glucagon (red) was performed at multiple levels before or 10 d after inducing apoptosis (C and D). GcgR mAb or IgG control was provided 3 d prior to dimerizer (E–G). Glucose (E) was measured before antibody and 10 d after dimerizer. Pancreatic sections were visualized by immunofluorescence against insulin (green) and glucagon (red) (F), and whole-islet area was quantified from multiple nonadjacent sections (G). n = 6. *P < 0.05, **P < 0.005. Standard error shown.