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. 2021 Feb 24;118(9):e2019194118. doi: 10.1073/pnas.2019194118

Fig. 5.

Fig. 5.

Np65 but not Np55 is required for LTP maintenance. (A and C) Plots show the mean ± SEM. AMPAR-EPSC amplitude of control (gray) and transfected (red) CA1 pyramid neurons normalized to the mean AMPAR-EPSC amplitude before LTP induction (arrow). Experiments were performed in neurons transfected with (A) CRISPR_Nptn + Np65 (control: n = 9; CRISPR_Nptn + Np65: n = 9; four mice; P = 0.8609 at 55.5 min) or (C) CRISPR_Nptn + Np55 (control: n = 11; CRISPR_Nptn + Np55: n = 11; five mice; ***P < 0.001 at 55.5 min). Representative AMPAR-EPSC current traces from control (gray) and transfected (red) neurons before and after LTP are shown to the right of the graphs. (B and D) Scatterplots show the AMPAR-EPSC amplitudes of single pairs (open circles) of control and transfected neurons; filled circle represents the mean ± SEM amplitude. Insets show representative traces from control (gray) and transfected cells (red). The bar graphs to the right of the scatterplots show the mean AMPAR-EPSC amplitude ± SEM of neurons transfected with (B) CRISPR_Nptn + Np65 (control: 150 ± 8.52; CRISPR_Nptn + Np65: 146 ± 11.2; n = 16 pairs; four mice; P = 0.6322; ns: not significant) or (D) CRISPR_Nptn + Np55 (control: 219 ± 19.4; CRISPR_Nptn + Np55: 141 ± 16.4; n = 18 pairs; five mice; **P < 0.01). (Scale bars, 25 pA, 25 ms.)