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. 2021 Feb 23;118(9):e2025512118. doi: 10.1073/pnas.2025512118

Fig. 5.

Fig. 5.

ELF3 is regulated by an EVT-specific superenhancer. (A) ATAC-seq of JEG3, JAR, HeLa, BJ fibroblasts, and BeWo upstream and downstream of ELF3 gene. (B) Schematic diagram showing the location of the primers used for ELF3 ChIP-qPCR analysis. (C) Chromatin obtained from JEG3 was immunoprecipitated using antibodies against H3K27Ac, BRD4, MED1, and IgG, and analyzed by qPCR with specific primer sets as indicated. (D) BRD4 occupancy fold change at +500-bp and +1.7-kb region downstream of ELF3 gene with and without JQ1 treatment at 500 nM, 72 h by ChIP-qPCR. (E) H3K27Ac occupancy fold change at +500-bp and +1.7-kb region downstream of ELF3 gene with and without JQ1 treatment at 500 nM, 72 h by ChIP-qPCR. ELF3 and HLA-C mRNA fold change after 500 nM JQ1 treatment in (F) JEG3 for 72 h; ELF3 mRNA fold change after 500 nM JQ1 treatment in (G) primary EVTs for 30 h and (H) primary EVTs for 72 h. qPCR was performed in triplicate. Data are reported as the mean ± SD.