Fig. 1.
Electroporation and tracking of [Cy5]tRNAPhe in aminoglycoside-treated E. coli cells (A) Microscopy sample preparation. (B) Representation of one [Cy5]tRNAPhe-binding cycle. The fast diffusion state is assigned to freely diffusing tRNA and tRNA bound to EF-Tu, while the slow state represents ribosome-bound tRNA, comprising two consecutive elongation cycles. (C) Example of segmented E. coli cells and the generated trajectories from the fluorescent particle tracking, fitted using HMM, and course-grained to two diffusional states—fast and slow.