Fig. 2.
Longer and less frequent ribosome binding of [Cy5]tRNAPhe during aminoglycoside treatment. Single-molecule tracking of Phe-[Cy5]tRNAPhe in live E. coli cells, untreated or exposed to apramycin, gentamicin, and paromomycin at 10 and 100 µg/mL. Error bars represent bootstrap estimates of SEs. (A) HMM-estimated dwell-time of Phe-[Cy5]tRNAPhe in the ribosome-bound state. (B) HMM-estimated occupancy of Phe-[Cy5]tRNAPhe in the ribosome-bound state. (C) Estimated usage frequency of Phe-[Cy5]tRNAPhe calculated from the occupancy in the ribosome-bound state divided by the corresponding dwell-time.