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. 2021 Feb 22;118(9):e2013315118. doi: 10.1073/pnas.2013315118

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Copyright © 2021 the Author(s). Published by PNAS.

This open access article is distributed under Creative Commons Attribution License 4.0 (CC BY).

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Fig. 3. — Measurements of the effect of aminoglycosides in the first elongation cycle. Single-molecule tracking of fMet-[Cy5]tRNA^fMet in live E. coli cells, untreated or exposed to aminoglycoside antibiotics at 10 and 100 µg/mL Error bars represent bootstrap estimates of SEs. (A) HMM-estimated dwell-time of fMet-[Cy5]tRNA^fMet in the ribosome-bound state. (B) HMM-estimated occupancy of fMet-[Cy5]tRNA^fMet in the ribosome-bound state. (C) Usage frequency of fMet-[Cy5]tRNA^fMet calculated using the dwell-times and occupancies at the ribosome-bound state.