Fig. 5.
The impact of aminoglycosides in the first elongation cycle compared to an average elongation cycle. The first elongation cycle time was obtained by subtracting the subunit joining time (40 ms) calculated from [Cy5]tRNAfMet tracking in the absence of antibiotics from the [Cy5]tRNAfMet ribosome-bound dwell-time in cells exposed to the aminoglycosides. The average elongation time was calculated considering that the observed dwell-time of [Cy5]tRNAPhe in the ribosome-bound state spans two elongation cycles at any point in the mRNA. Error bars represent bootstrap estimates of SEs propagated from the dwell-time error-estimates.