Fig. 1.

Fluorescence responses and activity-based sensing and labeling properties of PG1-FM. (A) Activity-based sensing fluorescence responses of 1 μM PG1-FM to 25 μM H₂O₂. Data were acquired in phosphate-buffered saline (PBS) (pH 7.4) at 37 °C with 488 nm excitation. (B) Fluorescence responses of 1 μM PG1-FM toward biologically relevant competing ROS and reactive nitrogen species. Data were acquired in PBS (pH 7.4) at 37 °C with 488 nm excitation. (C) H₂O₂-dependent activity-based labeling of PG1-FM to BSA. A solution of BSA (0.5 mg/mL) and PG1-FM (1 μM) in PBS (pH 7.4) was incubated with or without H₂O₂ (100 μM) and analyzed by SDS-PAGE gel.