Fig. 3.

PG1-FM imaging of endogenous H₂O₂ generation in live A431 cells under redox signaling conditions with EGF stimulation. (A) Confocal microscopy (Top) and overlaid brightfield (Bottom) images of A431 cells stained with PG1-FM (10 μM) and then treated with EGF (1 μg/mL) or vehicle control for 30 min, washed and imaged, or first pretreated with Nox inhibitor DPI (5 μM) or antioxidant ebselen (5 μM) for 30 min in HBSS solution before PG1-FM staining and EGF stimulation. (B) Quantification of experiments. (Scale bar: 20 μm.) *P < 0.05.